TABLE 1. Induction of creatinine deiminase in Clostridium sp. strains XP32 and XP56
| N sourcea |
Clostridium sp. strain XP32
|
Clostridium sp. strain XP56
|
| Total enzymeb |
Sp act (U/mg of protein) |
Total enzyme |
Sp act (U/mg of protein) |
|
| Ammonia |
0.58 |
0.32 |
0.50 |
0.28 |
| Glutamic acid |
5.36 |
1.48 |
2.18 |
0.61 |
| Aspartic acid |
2.72 |
0.15 |
1.47 |
0.06 |
| Arginine |
3.58 |
2.18 |
3.38 |
2.19 |
| Creatinine |
97.30 |
58.40 |
104.00 |
58.30 |
|
a The inoculum was grown in glucose broth with ammonium sulfate, washed twice, and then transferred into the media with the N sources listed above.
b Enzyme units in cell extract obtained from ca. 1010 cells.