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J Bacteriol. 1969 October; 100(1): 1-4
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Specificity and Evolutionary Divergence of the Antigenic Structure of the Polypeptide Chain Elongation Factors

¹ The Rockefeller University, New York, New York 10021

ABSTRACT

Antisera have been prepared against two electrophoretically homogeneous "polypeptide chain elongation factors," T and G, from Escherichia coli. Inactivation and precipitation tests showed that these two fractions were antigenically distinct with no cross-reaction. The immune inactivation curve of G factor from E. coli was distinguishable from that of G factor from Pseudomonas fluorescens. Mammalian factors were not inhibited by antibody directed against the E. coli T and G factors. By Ouchterlony diffusion tests, the antisera also detected significant antigenic variability among different bacterial species. It is concluded that the factors have undergone considerable evolutionary divergence in their antigenic structure.