This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Loomis, W. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Loomis, W. F., Jr.

 Previous Article  |  Next Article 

J Bacteriol. 1969 October; 100(1): 417-422
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Developmental Regulation of Alkaline Phosphatase in Dictyostelium discoideum

William F. Loomis Jr.

Department of Biology, University of California, San Diego, La Jolla, California 92037

ABSTRACT

The kinetics of accumulation of alkaline phosphatase activity were determined in cells of wild-type and morphologically aberrant mutant strains of Dictyostelium discoideum induced to develop synchronously on membrane supports. The enzyme specific activity increased slowly in wild-type cells until culmination when a dramatic rise in specific activity occurred. The patterns of accumulation in the mutant strains, as well as previous electrophoretic analysis, suggest that the two phases of accumulation may result from the synthesis of distinct isozymes. The rapid accumulation of alkaline phosphatase was found to require concomitant protein synthesis. Ribonucleic acid synthesis, on the other hand, could be inhibited during the 8 hr immediately preceding culmination without affecting the amount of enzyme accumulated. When ribonucleic acid synthesis was inhibited earlier in development, the accumulation of alkaline phosphatase was reduced. Comparison of these results with work on other developmentally controlled enzymes suggests that both transcriptional and translational control occurs during development of D. discoideum. The accumulation of alkaline phosphatase was shown to require specific cellular topography during culmination, suggesting that intercellular interactions which allow synthesis of alkaline phosphatase occur at that stage.

J Bacteriol. 1969 October; 100(1): 417-422
Copyright © 1969 American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Gloss, A., Rivero, F., Khaire, N., Muller, R., Loomis, W. F., Schleicher, M., Noegel, A. A. (2003). Villidin, a Novel WD-repeat and Villin-related Protein from Dictyostelium, Is Associated with Membranes and the Cytoskeleton. Mol. Biol. Cell 14: 2716-2727 [Abstract] [Full Text]  
  • Janssen, K.-P., Rost, R., Eichinger, L., Schleicher, M. (2001). Characterization of CD36/LIMPII Homologues in Dictyostelium discoideum. J. Biol. Chem. 276: 38899-38910 [Abstract] [Full Text]  
  • Srinivasan, S., Alexander, H., Alexander, S. (1999). The Prespore Vesicles of Dictyostelium discoideum. PURIFICATION, CHARACTERIZATION, AND DEVELOPMENTAL REGULATION. J. Biol. Chem. 274: 35823-35831 [Abstract] [Full Text]  
  • Rivero, F., Kuspa, A., Brokamp, R., Matzner, M., Noegel, A. A. (1998). Interaptin, an Actin-binding Protein of the {alpha}-Actinin Superfamily in Dictyostelium discoideum, Is Developmentally and cAMP-regulated and Associates with Intracellular Membrane Compartments. JCB 142: 735-750 [Abstract] [Full Text]  
  • Doring, V, Veretout, F, Albrecht, R, Muhlbauer, B, Schlatterer, C, Schleicher, M, Noegel, A. (1995). The in vivo role of annexin VII (synexin): characterization of an annexin VII-deficient Dictyostelium mutant indicates an involvement in Ca(2+)-regulated processes. J. Cell Sci. 108: 2065-2076 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»