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J Bacteriol. 1969 October; 100(1): 526-527
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Streptolysin O: Sedimentation Coefficient and Molecular Weight Determinations

¹ Department of Microbiology, Northwestern University School of Medicine, Chicago, Illinois 60611
Department of Medicine, Northwestern University School of Medicine, Chicago, Illinois 60611

ABSTRACT

The sedimentation coefficient of streptolysin O as determined by sucrose density gradient ultracentrifugation is 3.7S. An approximate molecular weight of 60,500 was calculated from the sedimentation velocity, and a similar value was obtained by Sephadex gel filtration. There was no measurable difference in the sedimentation coefficient of streptolysin O in either the active or reversibly inactive forms, indicating that there were at most only minor conformational differences between the two forms.