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J Bacteriol. 1969 October; 100(1): 86-94
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Ileal Loop Fluid Accumulation and Production of Diarrhea in Rabbits by Cell-free Products of Clostridium perfringens¹

^a Food Research Institute, Department of Bacteriology, and Department of Food Science, University of Wisconsin, Madison, Wisconsin 53706

ABSTRACT

The ability of cell extracts and culture filtrates of various strains of C. perfringens to produce ileal loop fluid accumulation and overt diarrhea in rabbits was tested. Good correlation was obtained in the ability of whole cells and a toxic factor (present in cell extracts and concentrated culture filtrates) to produce both fluid accumulation in ileal loops and diarrhea when injected into the normal ileum of the rabbit. The toxic factor was present in cell-free preparations when cells were grown in a sporulation medium, but not when they were grown in an asporogenic medium. The factor was shown to be heat labile, nondialyzable, and was inactivated by Pronase but not by trypsin, lipase, or amylase. Loss of activity occurred at pH 1.0, 3.0, 5.0, and 12.0.

¹ Presented in part at the 69th Annual Meeting of the American Society for Microbiology, 4–9 May 1969. Published with the permission of the Director of the Wisconsin Agricultural Experiment Station, Madison.

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