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J Bacteriol. 1969 November; 100(2): 1083-1090
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Surface Localization of Escherichia coli 5'-Nucleotidase by Electron Microscopy

¹ Department of Urology and Medicine, College of Physicians and Surgeons, Columbia University, New York, New York 10032

ABSTRACT

The 5'-nucleotidase of Escherichia coli was shown to be located at the cell wall surface by histochemical techniques utilizing the deposition of inorganic phosphate. Penetration of the 5'-nucleotidase in the periplasmic space was seen only in cells treated with ethylenediaminetetraacetic acid (EDTA)-tris(hydroxymethyl)aminomethane (Tris). The 3'-nucleotidase of E. coli was also found to have a surface location, and periplasmic precipitation of inorganic phosphate was seen only after EDTA-Tris-sucrose exposure.