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J Bacteriol. 1969 November; 100(2): 601-604
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Evidence for Two Genes Specifically Involved in Unsaturated Fatty Acid Biosynthesis in Escherichia coli

¹ Department of Biological Chemistry, Washington University School of Medicine, St. Louis, Missouri 63110

ABSTRACT

Unsaturated fatty acid auxotrophs of Eschericha coli have been divided into two distinct cistrons by extract complementation and genetic complementation based on abortive transduction. Lesions in one cistron result in the loss of the ß-hydroxydecanoyl thioester dehydrase which produces the first biosynthetic intermediate in unsaturated fatty acid formation. Evidence is presented which indicates that lesions in the second cistron result in the lack of a second enzyme specifically involved in the biosynthesis of unsaturated fatty acids.

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