This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Golub, E. E. Articles by Nishimura, J. S. Search for Related Content PubMed PubMed Citation Articles by Golub, E. E. Articles by Nishimura, J. S.

 Previous Article  |  Next Article 

J Bacteriol. 1969 November; 100(2): 977-984
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Biosynthesis of the Actinomycin Chromophore: Incorporation of 3-Hydroxy-4-Methylanthranilic Acid into Actinomycins by Streptomyces antibioticus

^a Department of Biochemistry, Tufts University School of Medicine, Boston, Massachusetts 02111

ABSTRACT

Actinomycin synthesis by washed mycelia of Streptomyces antibioticus has been conducted in the presence of 3-hydroxy-4-methylanthranilate-(carboxyl-¹⁴C). Incorporation of this compound into actinomycins has been observed, which constitutes further evidence that 3-hydroxy-4-methylanthranilate is an intermediate in actinomycin biosynthesis. The position of the incorporated label has been determined to be within the actinomycin chromophore, and the label appears to be equally distributed between both halves of the chromophore. Incidental to these findings was the observation that the ¹⁴C-labeled actinomycins were subject to rapid reabsorption by the organism with actinomycin V taken up preferentially to actinomycin IV.

¹ Present address: Division of Biology, California Institute of Technology, Pasadena, Calif. 91109.

² Present address: Department of Biochemistry, University of Texas Medical School, San Antonio, Tex. 78229.