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J Bacteriol. 1970 July; 103(1): 178-183
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Mutants of Escherichia coli K-12 with an Altered Glutamyl-Transfer Ribonucleic Acid Synthetase

E. J. Murgola1 and E. A. Adelberg

a Department of Microbiology, Yale University, New Haven, Connecticut 06510

ABSTRACT

Three streptomycin-suppressible lethal mutants of Escherichia coli K-12 have been shown to possess structurally altered glutamyl-transfer ribonucleic acid (tRNA) synthetases. Each mutant synthetase displays a Km value for glutamate which is 10-fold higher than the parental value, and the mutations reside in two widely separate loci on the genetic map. Mixing of the mutant extracts in pairs gave no indication of in vitro complementation. All three enzymes charge the minor tRNAglu fraction identically, but one (EM 120) charges the major fraction at a twofold lower rate than do the other two (EM 102 and EM 111). Possible explanations for the existence of the two synthetase loci are presented.

FOOTNOTES

1 Predoctoral trainee of the U.S. Public Health Service. The material in this paper is taken in part from a dissertation presented to the Graduate School of Yale University by E. J. Murgola in partial fulfillment of the requirements for the Ph.D. degree.

Present address: Department of Biological Sciences, Stanford University, Stanford, Calif. 94305.

J Bacteriol. 1970 July; 103(1): 178-183
Copyright © 1970 American Society for Microbiology. All Rights Reserved.



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