This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Turner, J. R. Articles by Matchett, W. H. Search for Related Content PubMed PubMed Citation Articles by Turner, J. R. Articles by Matchett, W. H.

Temporal Separation of Transcription and Translation in Neurospora

^a Biology Department, Battelle Memorial Institute, Pacific Northwest Laboratories, Richland, Washington 99352

ABSTRACT

Cells of Neurospora accumulated capacity for synthesis of kynureninase in the presence of cycloheximide and a suitable inducer. The capacity was expressed only when the cells were returned to a medium which allowed net synthesis of protein to resume. Subsequent expression did not require synthesis of ribonucleic acid (i.e., was not inhibited by actinomycin D) or the presence of inducer. The relative level of capacity, as judged by the initial rate of appearance of enzyme, increased linearly for 70 min in cells incubated in the presence of inducer plus antibiotic.

¹ Present address: Department of Biochemistry, University of Kansas, Lawrence, Kan. 66045.

² Present address: Battelle Seattle Research Center, Battelle Memorial Institute, Seattle, Wash. 98105.