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Relationship Between Radiation Response and the Deoxyribonucleic Acid Replication Cycle in Bacteria: Dependence on the Excision-Repair System

¹ Radiation Biology Laboratory, Division of Nuclear Sciences, and Departments of Microbiology and Radiology College of Medicine, University of Florida, Gainesville, Florida 32601

ABSTRACT

Prestarvation of Escherichia coli for required amino acids results in a marked enhancement in both ultraviolet light (UV) or X-ray resistance for selective strains. Preventing protein synthesis by starvation for required amino acids results in completion of the cycle of chromosomal replication then underway. We have investigated the relationship between starvation-induced resistance enhancement (SIRE) and the excision-repair (Hcr) system in several E. coli strains including E. coli B/r hcr⁺ and its isogenic mutant E. coli B/r hcr^–. The following observations were made. (i) The Hcr system is the major component of SIRE in UV-irradiated strain B/r. By using the Hcr⁺ strain, SIRE increases the 10% survival dose from 400 ergs to 1,200 ergs/mm². With the Hcr cells, the increase is from 45 ergs to 60 ergs/mm². (ii) Although prestarvation leads to a moderate enhancement of resistance to X irradiation, this effect is not dependent on the Hcr system. (iii) The double mutant, E. coli B_s–1 (hcr^–exr^–) is completely unable to express SIRE whether studied with UV or X irradiation. It is concluded that the Hcr system is the major system responsible for SIRE in UV-treated cells, whereas Exr (resistance to X rays) may be involved to a minor extent. The Exr character appears to be required for SIRE expression in X-ray exposed cells.

This article has been cited by other articles:

• Blaisdell, J. O., Wallace, S. S. (2001). Abortive base-excision repair of radiation-induced clustered DNA lesions in Escherichia coli. Proc. Natl. Acad. Sci. USA 98: 7426-7430 [Abstract] [Full Text]