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J Bacteriol. 1970 August; 103(2): 435-438
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Relationship of Nuclear Segregation and Macroconidial Germination in Microsporum gypseum¹

^a Department of Microbiology, The University of British Columbia, Vancouver 8, British Columbia, Canada

ABSTRACT

Microsporum gypseum macroconidia germinated at 37 C possessed from one to eight nuclei per germinated spore compartment. The distribution of nuclei per spore compartment was the result of a random packaging of nuclei from the available nuclear population. Partial inhibition of germination by incubation at 25 C or at 37 C in the presence of 10^–4M phenyl methyl sulfonyl-fluoride resulted in an enrichment of germinated spores containing high numbers of nuclei per compartment. The selection for higher nuclear numbers was statistically significant. Compartments possessing high numbers of nuclei appeared to be precommitted to spore germination since they were not sensitive to germination inhibition. The effect of incubation temperature variation on spore germination is discussed with respect to the organism's natural environment.

² Present address: Department of Biochemistry and Biophysics, University of California, Davis, Calif. 95616.

¹ A preliminary report covering part of this work was presented at the 70th Annual Meeting of the American Society for Microbiology, Boston, Mass., 26 April–1 May 1970.