This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Fan, D. P. Search for Related Content PubMed PubMed Citation Articles by Fan, D. P.

Cell Wall Binding Properties of the Bacillus subtilis Autolysin(s)

¹ Department of Genetics and Cell Biology, University of Minnesota, St. Paul, Minnesota 55101, and Laboratoire Biophysique, University of Geneva, Geneva, Switzerland

ABSTRACT

Cell walls isolated from exponentially growing Bacillus subtilis have autolysin(s) attached to them. An autolysin can be released from the walls by incubation at 0 C with 3 M LiCl. The enzyme can reattach to walls when the salt concentration is reduced. The bound enzyme cannot be removed or destroyed by washing the walls with 8 M urea at 0 C. The binding of free enzyme to walls at 0 C can take place normally in the presence of 2 M urea.