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J Bacteriol. 1970 December; 104(3): 1280-1285
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Periodic Density Fluctuation During the Yeast Cell Cycle and the Selection of Synchronous Cultures

¹ Department of Genetics, University of Washington, Seattle, Washington 98105

ABSTRACT

Yeast cells undergo periodic fluctuations in density during the cell division cycle such that a minimum in density occurs at the time of cell separation whereas a maximum occurs between the time of deoxyribonucleic acid replication and nuclear division. Synchronous cultures can be selected from asynchronously growing cell cultures by withdrawing the cells of least or greatest density after banding in Renografin-sucrose density gradients. This technique is rapid, reproducible, and almost unlimited in capacity.