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J Bacteriol. 1970 December; 104(3): 1299-1306
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Manganese Active Transport in Escherichia coli

¹ Department of Biology, Washington University, St. Louis, Missouri 63130

ABSTRACT

Manganese was accumulated by cells of Escherichia coli by means of an active transport system quite independent of the magnesium transport system. When the radioisotope ⁵⁴Mn was used, manganese transport showed saturation kinetics with a K_m of 2 x 10^–7M and a V_max of 1 to 4 nmoles/min per 10¹² cells at 25 C. The manganese transport system is highly specific; magnesium and calcium did not stimulate, inhibit, or compete with manganese for cellular uptake. Cobalt and iron specifically interfered with ⁵⁴Mn uptake, but only when added at concentrations 100 times higher than the K_m for manganese. Active transport of manganese is temperature- and energy-dependent: uptake of ⁵⁴Mn was inhibited by cyanide, dinitrophenol, and m-chlorophenyl carbonylcyanide hydrazone (CCCP). Furthermore, the turnover or exit of manganese from intact cells was inhibited by energy poisons such as dinitrophenol and CCCP.

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