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J Bacteriol. 1971 January; 105(1): 142-149
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Gene Dosage for 5S Ribosomal Ribonucleic Acid in Escherichia coli and Bacillus megaterium¹

^a Division of Research, National Jewish Hospital and Research Center, and Department of Biophysics, University of Colorado School of Medicine, Denver, Colorado 80206

ABSTRACT

The number of gene copies for 5S ribosomal ribonucleic acid (rRNA), relative to that for 16 and 23S rRNA, has been determined by deoxyribonucleic acid (DNA)-RNA hybridization for Escherichia coli and Bacillus megaterium. In both cases, the number of 5S rRNA genes equals the number of 16 or 23S rRNA genes. Rapid procedures for preparing extremely highly purified DNA suitable for DNA-RNA hybridization experiments and chemically pure 5S rRNA are described.

¹ Department of Biophysics Publication no. 427.