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Effects of Essential Amino Acid Starvation in Streptococcus faecalis: Structural Change in the 50S Ribosomal Subunit

^a Department of Microbiology and Immunology, Temple University Health Sciences Center, Philadelphia, Pennsylvania 19140

ABSTRACT

The 50S ribosomal subunits obtained from exponential-phase and valine-limited cultures of Streptococcus faecalis 9790 differed in protein content. Approximately 35% of the valine-limited subunits showed protein losses when analyzed by CsCl density centrifugation after formaldehyde fixation. Results of density gradient analysis and of acrylamide gel electrophoresis localized the protein to a "split" subunit fraction. No differences could be detected in the protein contents of 30S subunits from exponential-phase and valine-limited cultures.

¹ Present address: The Rockefeller University, New York, N.Y.