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J Bacteriol. 1971 January; 105(1): 376-383
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Purification of Intact Flagella from Escherichia coli and Bacillus subtilis

Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706
Department of Genetics, University of Wisconsin, Madison, Wisconsin 53706

ABSTRACT

A procedure is described for the purification of bacterial flagella in the form of a filament-hook-basal body complex (intact flagella) free from detectable cell wall, membrane, or cytoplasmic material. Spheroplasts produced with lysozyme and ethylenediaminetetraacetic acid were lysed with Triton X-100, and the flagella were purified by (NH₄)₂SO₄ precipitation, differential centrifugation, and CsCl gradient centrifugation. As much as 40% of the flagella were recovered, and they contained about one basal body per 4 to 6 µm of flagella. The same procedure developed for Escherichia coli was also successful for purifying intact flagella from Bacillus subtilis.

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