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Mutant of Escherichia coli K-12 Defective in D-Glucosamine Biosynthesis

^a Department of Bacteriology and Immunology, University of California, Berkeley, California 94720

ABSTRACT

A mutant was isolated from a derivative of Escherichia coli K-12 after mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine. This mutant contained normal levels of 2-amino-2-deoxy-D-glucose-6-phosphate ketol-isomerase (deaminating) (EC 5.3.1.10), but no detectable activity of L-glutamine:D-fructose-6-phosphate amino-transferase (EC 2.6.1.16). It required either N-acetyl-D-glucosamine or D-glucosamine for growth, and went into rapid lysis when the supply of these compounds was exhausted. In medium containing 11% sucrose, the cells were converted into spheroplasts in the absence of D-glucosamine.

¹ Present address: State Serum Institute, Helsinki, Finland.

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