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J Bacteriol. 1971 February; 105(2): 573-579
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Microcalorimetric Determination of the Affinity of Saccharomyces cerevisiae for Some Carbohydrate Growth Substrates

M. Murgier and J. P. Belaich

Laboratoire de Chimie Bactérienne, C.N.R.S., Marsielle, France

ABSTRACT

A microcalorimetric method using a Tian-Calvet apparatus was utilized for measuring the Km and Vmax of intact yeast cells for some carbohydrate substrates. These values were obtained for fructose, galactose, and maltose. The heats of ethanolic fermentations were also directly determined, and the results were very close to those obtained by using theoretical calculations and standard values.

J Bacteriol. 1971 February; 105(2): 573-579
Copyright © 1971 American Society for Microbiology. All Rights Reserved.





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Appl. Environ. Microbiol. Infect. Immun. Eukaryot. Cell
Mol. Cell. Biol. J. Virol. Microbiol. Mol. Biol. Rev.
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Copyright © 1971 by the American Society for Microbiology. All rights reserved.