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J Bacteriol. 1971 February; 105(2): 629-636
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Mutant of Bacillus subtilis Demonstrating the Requirement of Lysis for Growth

David P. Fan and Mary M. Beckman

1 Department Genetics and Cell Biology, University of Minnesota, St. Paul, Minnesota 55101

ABSTRACT

A temperature-sensitive mutant of Bacillus subtilis which has impaired ability to autolyze was isolated. The growth rate of mutant cells at high temperature can be increased by the addition of egg-white lysozyme or a B. subtilis autolysin. Experiments with this mutant show that lysis is essential for cell growth.

J Bacteriol. 1971 February; 105(2): 629-636
Copyright © 1971 American Society for Microbiology. All Rights Reserved.



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