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J Bacteriol. 1971 March; 105(3): 1090-1098
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Mechanism of Excretion of a Bacterial Proteinase: Demonstration of Two Proteolytic Enzymes Produced by a Sarcina Strain (Coccus P)

^a Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115

ABSTRACT

A Sarcina strain (Coccus P) produces two proteolytic enzymes. One is found only extracellularly, is far more prevalent, and is actively excreted during exponential growth. It is the enzyme responsible for the known strong proteolytic activity of the cultures of this strain. A second protease is, however, produced which remains associated with the intact cells but is released by the protoplasts. The two enzymes appear unrelated in their derivation. Calcium ions play an essential role in preventing autodigestion of the excreted enzyme.

¹ Present address: Biophysics Department, Portsmouth Polytechnic, Portsmouth, England.

² Present address: Department of Molecular Biology, University of California, Berkeley.

³ Present address: Laboratorio Internazionale di Genetica e Biofisica, Naples, Italy.