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Division Cycle of Myxococcus xanthus III. Kinetics of Cell Growth and Protein Synthesis

Department of Bacteriology and Institute of Molecular Biology, University of California, Los Angeles 90024
Jet Propulsion Laboratory, California Institute of Technology, Pasadena, California 91103

ABSTRACT

The kinetics of cell growth and protein synthesis during the division cycle of Myxococcus xanthus was determined. The distribution of cell size for both septated and nonseptated bacteria was obtained by direct measurement of the lengths of 8,000 cells. The Collins-Richmond equation was modified to consider bacterial growth in two phases: growth and division. From the derived equation, the growth rate of individual cells was computed as a function of size. Nondividing cells (growth phase) comprised 91% of the population and took up 87% of the time of the division cycle. The absolute and specific growth rates of nondividing cells were observed to increase continually throughout the growth phase; the growth rate of dividing cells could not be determined accurately by this technique because of changes in the geometry of cells between the time of septation and physical separation. The rate of protein synthesis during the division cycle was measured by pulselabeling an exponential-phase culture with radio-active valine or arginine and then preparing the cells for quantitative autoradiography. By measuring the size of individual cells as well as the number of grains, the rate of protein synthesis as a function of cell size was obtained. Nondividing cells showed an increase in both the absolute and specific rates of protein synthesis throughout the growth phase; the specific rate of protein synthesis for dividing cells was low when compared to growthphase cells. Cell growth and protein synthesis are compared to the previously reported kinetics of deoxyribonucleic acid and ribonucleic acid synthesis during the division cycle.

¹ Present address: Department of Biochemical Sciences, Moffett Laboratories, Princeton University, Princeton, N.J. 18540.

² Present address: Microbiology Department, Tel-Aviv University, Tel-Aviv, Israel.

This article has been cited by other articles:

• Rosario, C. J., Singer, M. (2007). The Myxococcus xanthus Developmental Program Can Be Delayed by Inhibition of DNA Replication. J. Bacteriol. 189: 8793-8800 [Abstract] [Full Text]  
• Harris, B. Z., Kaiser, D., Singer, M. (1998). The guanosine nucleotide (p)ppGpp initiates development and A-factor production in Myxococcus xanthus. Genes Dev. 12: 1022-1035 [Abstract] [Full Text]