JB
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bukhari, A. I.
Right arrow Articles by Taylor, A. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bukhari, A. I.
Right arrow Articles by Taylor, A. L.

 Previous Article  |  Next Article 

J Bacteriol. 1971 March; 105(3): 844-854
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Genetic Analysis of Diaminopimelic Acid- and Lysine-Requiring Mutants of Escherichia coli1

Ahmad I. Bukhari2 and Austin L. Taylor

a Department of Microbiology, University of Colorado Medical Center, Denver, Colorado 80220

ABSTRACT

Several diaminopimelic acid (DAP)- and lysine-requiring mutants of Escherichia coli were isolated and studied by genetic, physiological, and biochemical means. The genes concerned with DAP-lysine synthesis map at several different sites on the E. coli chromosome and, therefore, do not constitute a single operon. Three separate loci affecting DAP synthesis are located in the 0 to 2.5 min region of the genetic map. The order of the loci in this region is thr-dapB-pyrA-ara-leu-pan-dapC-tonA-dapD. Two additional DAP genes map in the region between min 47 and 48, with the gene order being gua-dapA-dapE-ctr. The lys locus at min 55 determines the synthesis of the enzyme DAP decarboxylase, which catalyzes the conversion of DAP into lysine. The order of the genes in this region is serA-lysA-thyA.

FOOTNOTES

2 Present address: Cold Spring Harbor Laboratory for Quantitative Biology, Cold Spring Harbor, N.Y. 11724.

1 This work was presented in part at the 68th Annual Meeting of the American Society for Microbiology, Miami Beach, Fla., 4–9 May 1969. Submitted in partial fulfillment of the requirement for Ph.D. degree at the Department of Microbiology, University of Colorado Medical Center, Denver, C.

J Bacteriol. 1971 March; 105(3): 844-854
Copyright © 1971 American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Appl. Environ. Microbiol. Infect. Immun. Eukaryot. Cell
Mol. Cell. Biol. J. Virol. Microbiol. Mol. Biol. Rev.
ALL ASM JOURNALS

Copyright © 1971 by the American Society for Microbiology. All rights reserved.