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J Bacteriol. 1971 June; 106(3): 848-855
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Isolation and Characterization of Tricarboxylic Acid Cycle Mutants of Bacillus subtilis

^a Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706

ABSTRACT

A technique was developed for the detection, on agar, of mutants of Bacillus subtilis that lacked a functional tricarboxylic acid cycle. Mutants devoid of detectable levels of aconitase, isocitric dehydrogenase, alpha-ketoglutarate dehydrogenase, succinic dehydrogenase, fumarase, and malate dehydrogenase have been isolated and characterized. Several mutants with conditionally expressible lesions, including a mutant with a heat-sensitive citrate synthase, have also been isolated. All of the mutants examined express all the biochemical markers normally absent in early-stage sporulation mutants except elastase, and some of these mutants sporulated nearly as well as the prototroph.

¹ Present address: Department of Biology, Edinboro State College, Edinboro, Pa. 16412.

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