This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Beaman, T. C. Articles by Gerhardt, P. Search for Related Content PubMed PubMed Citation Articles by Beaman, T. C. Articles by Gerhardt, P.

 Previous Article  |  Next Article 

J Bacteriol. 1971 July; 107(1): 320-324
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Paracrystalline Sheets Reaggregated from Solubilized Exosporium of Bacillus cereus

Department of Microbiology and Public Health, Michigan State University, East Lansing, Michigan 48823

ABSTRACT

Fragments of exosporium, isolated from dormant spores of Bacillus cereus, were disintegrated by treatment with sodium dodecyl sulfate (SDS) or with phenol and acetic acid. After centrifugation of each preparation, proteins in the supernatant fractions were resolved by disc gel electrophoresis into either two or eight bands, respectively. The SDS-solubilized fraction contained spheroidal particles 11 to 44 nm in diameter. When centrifuged until clear, this fraction after dialysis still gave rise to crystal-like sheets which had the same lattice symmetry and major chemical components (protein, lipid, and carbohydrate) as fragments of the native exosporium.

This article has been cited by other articles:

• Thompson, B. M., Waller, L. N., Fox, K. F., Fox, A., Stewart, G. C. (2007). The BclB Glycoprotein of Bacillus anthracis Is Involved in Exosporium Integrity. J. Bacteriol. 189: 6704-6713 [Abstract] [Full Text]  
• Sylvestre, P., Couture-Tosi, E., Mock, M. (2005). Contribution of ExsFA and ExsFB Proteins to the Localization of BclA on the Spore Surface and to the Stability of the Bacillus anthracis Exosporium. J. Bacteriol. 187: 5122-5128 [Abstract] [Full Text]  
• Daubenspeck, J. M., Zeng, H., Chen, P., Dong, S., Steichen, C. T., Krishna, N. R., Pritchard, D. G., Turnbough, C. L. Jr. (2004). Novel Oligosaccharide Side Chains of the Collagen-like Region of BclA, the Major Glycoprotein of the Bacillus anthracis Exosporium. J. Biol. Chem. 279: 30945-30953 [Abstract] [Full Text]  
• Redmond, C., Baillie, L. W. J., Hibbs, S., Moir, A. J. G., Moir, A. (2004). Identification of proteins in the exosporium of Bacillus anthracis. Microbiology 150: 355-363 [Abstract] [Full Text]  
• Todd, S. J., Moir, A. J. G., Johnson, M. J., Moir, A. (2003). Genes of Bacillus cereus and Bacillus anthracis Encoding Proteins of the Exosporium. J. Bacteriol. 185: 3373-3378 [Abstract] [Full Text]  
• Steichen, C., Chen, P., Kearney, J. F., Turnbough, C. L. Jr. (2003). Identification of the Immunodominant Protein and Other Proteins of the Bacillus anthracis Exosporium. J. Bacteriol. 185: 1903-1910 [Abstract] [Full Text]  
• Sylvestre, P., Couture-Tosi, E., Mock, M. (2003). Polymorphism in the Collagen-Like Region of the Bacillus anthracis BclA Protein Leads to Variation in Exosporium Filament Length. J. Bacteriol. 185: 1555-1563 [Abstract] [Full Text]  
• Welkos, S., Little, S., Friedlander, A., Fritz, D., Fellows, P. (2001). The role of antibodies to Bacillus anthracis and anthrax toxin components in inhibiting the early stages of infection by anthrax spores. Microbiology 147: 1677-1685 [Abstract] [Full Text]