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J Bacteriol. 1971 August; 107(2): 499-504
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Staphylococcal Bacteriophage-Associated Lysin: a Lytic Agent Active Against Staphylococcus aureus

^a Department of Microbiology, Hahnemann Medical College, Philadelphia, Pennsylvania 19102

ABSTRACT

A lytic enzyme active against viable, intact staphylococci is released into culture fluids upon lysis of bacteriophage-infected Staphylococcus aureus PS53 cells. This enzyme, staphylococcal phage-associated lysin (PAL), was partially purified by ammonium sulfate precipitation and gel filtration through Sephadex G-200. PAL is optimally active at pH 6.5 and 30 C, and lytic activity is greatly enhanced by the addition of reducing agents. Lytic activity was observed against all strains of staphylococci tested and against purified staphylococcal cell walls, but no activity was noted against other bacterial species. PAL possesses peptidase activity and results in the production of spheroplasts which can be osmotically stabilized for extended periods by the addition of 7.5% polyethylene glycol 4000.

¹ Present address: Department of Microbiology, University of Georgia, Athens, Ga.

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