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Location of Three Genes Concerned with the Conversion of 2, 3-Dihydroxybenzoate into Enterochelin in Escherichia coli K-12

^a Department of Biochemistry, John Curtin School of Medical Research, The Institute of Advanced Studies, Australian National University, Canberra, A.C.T., Australia

ABSTRACT

Mutants of Escherichia coli K-12 unable to synthesize the iron-sequestering compound, enterochelin, from 2,3-dihydroxybenzoate have been isolated and divided into three classes on the basis of tests for enzymatic complementation. The genes affected (designated entD, entE, and entF) have been mapped by cotransduction and are located at about minute 14 on the E. coli genome. They were found to be closely linked to other genes (entA, entB, and entC) concerned with enterochelin biosynthesis and a gene (fep) concerned with the uptake of the iron-enterochelin complex. No detectable diffusible intermediate in the formation of enterochelin from 2,3-dihydroxybenzoate was formed by cell extracts of mutants carrying mutations in the entD, entE, or entF genes.

¹ Present address: School of Agriculture, La Trobe University, Victoria 3083, Australia.

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