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Identification of the Forms of Vitamin B₆ Present in the Culture Media of "Vitamin B₆ Control" Mutants

¹ Microbiology, Genetics and Biochemistry Unit, Veterans Administration Hospital, and Department of Biochemistry, The University of Texas Southwestern Medical School, Dallas, Texas 75216

ABSTRACT

An Escherichia coli mutant resistant to isoniazid (WG497) contained 0.6 µmole of extracellular pyridoxamine and pyridoxamine phosphate in the early stationary phase. A suppressed lysine mutant (AT1024) contained 1.4 µmoles of pyridoxal phosphate under the same conditions. The internal concentration of vitamin B₆ was one-half of normal for AT1024 and increased fivefold for WG497.

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