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Mitomycin C and Temperature Induction of Colicin B in the Absence of Deoxyribonucleic Acid Synthesis

^a Division of Biology, The University of Texas at Dallas, Dallas, Texas 75230

ABSTRACT

An Escherichia coli K-12 strain and its mutant, temperature-sensitive in deoxyribonucleic acid (DNA) synthesis, were used as hosts for two different ColB factors (ColB3-K166 and ColB4-K98). Induction of either colicin occurred in both hosts in the presence of mitomycin C at 42 C. Induction of the temperature-sensitive colicinogenic hosts also occurred without mitomycin C at 42 C at which temperature no DNA synthesis was observed. Colicin synthesis was a nonlethal event in ColB4-K98⁺ cultures, whereas in cultures of ColB3-K166⁺ induction led to death of a large fraction of the population.

¹ Present address: Institut für Genetik, Universität Freiburg, West Germany.