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J Bacteriol. 1971 September; 107(3): 659-663
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

New Method for Extraction of Ribonucleic Acid and Polyribosomes from Schizosaccharomyces pombe

H. C. Birnboim

Biology and Health Physics Division, Atomic Energy of Canada Limited, Chalk River, Ontario, Canada

ABSTRACT

A new method for extracting ribosomal ribonucleic acid (RNA) and polyribosomes from Schizosaccharomyces pombe, a fission yeast, has been developed. Cells grown in the presence of 2-deoxyglucose were found to be more sensitive to the action of Glusulase; a short period of treatment (10 to 20 min) at 0 C was sufficient to convert the cells to osmotically sensitive structures. From these, ribosomal RNA and polyribosomes were readily prepared.

J Bacteriol. 1971 September; 107(3): 659-663
Copyright © 1971 American Society for Microbiology. All Rights Reserved.





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Appl. Environ. Microbiol. Infect. Immun. Eukaryot. Cell
Mol. Cell. Biol. J. Virol. Microbiol. Mol. Biol. Rev.
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Copyright © 1971 by the American Society for Microbiology. All rights reserved.