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J Bacteriol. 1971 September; 107(3): 683-689
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Location and Orientation of the phoA Locus on the Escherichia coli K-12 Linkage Map

¹ Division of Biological and Medical Sciences, Brown University Providence, Rhode Island 02912, and Research Institute for Microbial Diseases, Osaka University, Osaka, Japan

ABSTRACT

The order of gene loci in the phoA-phoR region of the Escherichia coli K-12 linkage map was demonstrated to be lac phoA proC phoR. The end of the phoA locus corresponding to the amino terminus of alkaline phosphatase was shown to be the end nearer proC. Translation (and transcription) of phoA is therefore in the anticlockwise direction relative to the conventional E. coli linkage map.