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New Suppressor in Escherichia coli¹

^a School of Microbiology, University of Melbourne, Australia

ABSTRACT

During the genetic mapping of a mutation in the pheS gene which confers temperature sensitivity on a strain of Escherichia coli K-12, an extragenic suppressor was discovered which restores ability to grow at the restrictive temperature. The suppressor, which has been named supQ, is cotransduced by bacteriophage P1 with the purE marker. SupQ does not suppress a number of amber or ochre mutations. SupQ^– is carried by the prototrophic Hfr Hayes strain AB259, and the presence of the supQ^– allele impairs the growth of this strain at 42 C.

² Present address: CSIRO Division of Food Research, Cannon Hill, Queensland, Australia.

³ Present address: Pasteur Institute, Paris.

¹ These results are taken from a Thesis submitted by R.R.B.R. in partial fulfillment of the requirements for a Ph.D. Degree.

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