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Isoleucine Hydroxamate, an Isoleucine Antagonist

Department of Applied Biochemistry, Chemical Research Laboratory, Tanabe Seiyaku Co., Ltd., Kashima-cho, Higashiyodogawa-ku, Osaka, Japan

ABSTRACT

Isoleucine hydroxamate (Ile·Hdx) was found to inhibit the growth of Serratia marcescens and to antagonize isoleucine. At a low concentration of Ile·Hdx, at which the growth of the wild strain was completely inhibited, the growth of an isoleucine auxotroph was not inhibited in the medium containing a limiting amount of D-threonine as the isoleucine source. At a higher concentration, this antagonist exhibited a considerable inhibitory effect on the growth of the auxotroph. Ile·Hdx showed the same inhibitory effect as isoleucine on L-threonine dehydratase activity at the concentrations 10 times those of isoleucine. Ile·Hdx caused also derepression of isoleucine-valine biosynthetic enzymes and the derepression was overcome by isoleucine. These results indicate the Ile·Hdx causes growth inhibition by its effects on isoleucine metabolism.