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J Bacteriol. 1971 October; 108(1): 132-136
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Formation of ß-Cyanoalanine by O-Acetylserine Sulfhydrylase

¹ Department of Biochemistry and Biophysics, University of California, Davis, California 95616

ABSTRACT

Cell-free extracts of Bacillus megaterium form ß-cyanoalanine (ß-CNA)-¹⁴C from Na¹⁴CN and L-cysteine, O-acetyl-L-serine or, to a lesser extent, L-serine. However, the presence of cyanide in the growth medium does not increase the capacity of cell extracts to catalyze the formation of ß-CNA from cysteine and cyanide. The formation of ß-CNA is readily detected in extracts of cells grown in synthetic media with sulfate or L-djenkolic acid as sulfur sources; such cells also exhibit an increased ability to form cysteine when compared with cells grown on cysteine as the sulfur source. ß-CNA formation could not be detected in extracts of cells grown on cysteine as the sulfur source. A 40-fold purification of the O-acetyl-serine sulfhydrylase resulted in the co-purification of the ß-CNA-forming activity. The sulfhydrylase and the ß-CNA-forming activity co-chromatographed on diethyl-aminoethyl cellulose and Sephadex G-100.

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