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On the Process of Cellular Division in Escherichia coli VI. Use of a Methocel-Autoradiographic Method for the Study of Cellular Division in Escherichia coli

¹ Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, and Institut Pasteur and Collège de France, Paris, France

ABSTRACT

A technique allowing chain formation by Escherichia coli for the analysis of the segregation of certain cellular components by autoradiographic methods has been developed. Deoxyribonucleic acid labeled with ³H-thymidine does not appear to segregate preferentially with one or another of the bacteria. Membrane lipids labeled with either ³H-oleate or ³H-glycerol are evenly distributed among all progeny cells. A similar pattern of distribution of cell wall material is observed when ³H-diaminopimelate is used as the marker.