This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Dempsey, W. B. Search for Related Content PubMed PubMed Citation Articles by Dempsey, W. B.

Control of Vitamin B₆ Biosynthesis in Escherichia coli¹

^a Basic Research Unit, Veterans Administration Hospital, and Department of Biochemistry, University of Texas Southwestern Medical School, Dallas, Texas 75216

ABSTRACT

Pyridoxineless mutants of Escherichia coli B which specifically require pyridoxal or pyridoxamine for growth can be divided into classes according to their growth responses in enriched media. Members of the slowest growing class synthesize vitamin B₆ at the fastest rates when starved for pyridoxal in glycerol minimal medium. After 80 min of synthesis at 4 x 10^–10 moles of vitamin B₆ per mg of cells per hr, the rate increases four- to fivefold and continues at the new rate for several hours. The shift to the new rate is prevented by chloramphenicol, thus suggesting that a derepression mechanism exists to control vitamin B₆ synthesis in addition to the previously discovered feedback control.

¹ Presented at the 71st Annual Meeting of the American Society for Microbiology, Minneapolis, Minn., 2–7 May 1971.

This article has been cited by other articles:

• Pease, A. J., Roa, B. R., Luo, W., Winkler, M. E. (2002). Positive Growth Rate-Dependent Regulation of the pdxA, ksgA, and pdxB Genes of Escherichia coli K-12. J. Bacteriol. 184: 1359-1369 [Abstract] [Full Text]  
• Osmani, A. H., May, G. S., Osmani, S. A. (1999). The Extremely Conserved pyroA Gene of Aspergillus nidulans Is Required for Pyridoxine Synthesis and Is Required Indirectly for Resistance to Photosensitizers. J. Biol. Chem. 274: 23565-23569 [Abstract] [Full Text]  
• Yang, Y., Tsui, H.-C. T., Man, T.-K., Winkler, M. E. (1998). Identification and Function of the pdxY Gene, Which Encodes a Novel Pyridoxal Kinase Involved in the Salvage Pathway of Pyridoxal 5'-Phosphate Biosynthesis in Escherichia coli K-12. J. Bacteriol. 180: 1814-1821 [Abstract] [Full Text]