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J Bacteriol. 1971 October; 108(1): 459-464
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Microbial Transformation of Antibiotics: Phosphorylation of Clindamycin by Streptomyces coelicolor Müller¹

^a Research Laboratories, the Upjohn Company, Kalamazoo, Michigan 49001

ABSTRACT

Addition of clindamycin to whole-cell cultures of Streptomyces coelicolor Müller resulted in the loss of in vitro activity against organisms sensitive to clindamycin. Incubation of such culture filtrates with alkaline phosphatase generated a biologically active material identified as clindamycin. Fermentation broths containing inactivated clindamycin yielded clindamycin 3-phosphate, the structure of which was established by physical-chemical and enzymatic studies. Clindamycin was phosphorylated by lysates and partially purified enzyme preparations from S. coelicolor Müller. These reactions require a ribonucleoside triphosphate and Mg²⁺. The product of the cell-free reactions was identified as clindamycin 3-phosphate.

¹ Presented in part at the 9th Interscience Conference on Antimicrobial Agents and Chemotherapy, Washington, D.C., 27 October 1969.