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Fate of Transforming Deoxyribonucleate in Bacillus subtilis

^a Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139

ABSTRACT

The majority of donor deoxyribonucleate (DNA) at early stages after uptake was found in a complex with a cell component which changes its buoyant behavior on equilibrium density gradients. Analysis of the recipient cell lysates, after treatment to dissociate the complex, showed about two-thirds of the donor molecules in denatured form and the rest associated with recipient DNA. Incubation of cells after DNA uptake leads to the disappearance of denatured donor DNA and to the increase of donor label associated with recipient DNA. Some characteristics of a component from intact cells or spheroplasts with affinity for denatured Bacillus subtilis DNA are described.

¹ Present address: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.

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