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J Bacteriol. 1971 November; 108(2): 751-756
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Oxidation of Nicotinic Acid by a Bacillus Species: Purification and Properties of Nicotinic Acid and 6-Hydroxynicotinic Acid Hydroxylases

^a Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706

ABSTRACT

The enzymes of a Bacillus species that hydroxylate nicotinic acid to 6-hydroxynicotinic acid and 6-hydroxynicotinic acid to 2,6-dihydroxynicotinic acid were purified and characterized. The purified enzymes contained approximately two molecules of flavine and eight molecules of iron per molecule of enzyme. The enzymes were large (molecular weight, 400,000 to 450,000) and appeared to consist of subunits.

¹ Present address: Department of Molecular Biology, Albert Einstein College of Medicine, Yeshiva University, Bronx, New York, N.Y. 10461.