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Oxidation of Nicotinic Acid by a Bacillus Species: Source of Oxygen Atoms for the Hydroxylation of Nicotinic Acid and 6-Hydroxynicotinic Acid

^a Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706

ABSTRACT

Three types of evidence are presented to show that the enzymes that hydroxylate nicotinic acid to 2,6-dihydroxynicotinic acid use water as a source of oxygen atoms. ¹⁸O is incorporated into the products from H₂¹⁸O. Molecular oxygen acts as a terminal electron acceptor, one-half molecule being consumed per molecule of hydroxyl groups incorporated. An external electron acceptor is required for activity in purified preparations.

¹ Present address: Department of Molecular Biology, Albert Einstein College of Medicine, Yeshiva University, Bronx, New York, N.Y. 10461.

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