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J Bacteriol. 1971 November; 108(2): 868-873
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Macromolecular Sieving by the Dormant Spore of Bacillus cereus1

Réne Scherrer, T. Cabrera Beaman and Philipp Gerhardt

a Department of Microbiology and Public Health, Michigan State University, East Lansing, Michigan 48823

ABSTRACT

The threshold surface porosity in the dormant spore of Bacillus cereus strain T was assessed by measuring passive permeabilities to a series of polydisperse polyethylene glycol samples which increased in average molecular size. The apparent exclusion threshold at diffusional equilibrium corresponded to a polymer of number-average molecular weight ({macron}Mn) = 150,000 and equivalent hydrodynamic radius ({macron}rES) = 16 nm, which confirmed a previous report. However, analytical gel chromatography before and after uptake by the spores revealed that only the low molecular weight fractions in a polymer sample distribution were taken up. From graphical analyses of the changes in molecular weight distributions, a quasi-monodisperse exclusion threshold was determined corresponding to Mn = 8,000 and rES = 3.2 nm. Thus, the equivalent porosity in the limiting outer integument appeared much more restrictive than heretofore shown for spores, although still more open than the monodisperse equivalent for the cell wall of vegetative bacilli.

FOOTNOTES

1 Journal article no. 5542 of the Michigan Agricultural Experiment Station.

J Bacteriol. 1971 November; 108(2): 868-873
Copyright © 1971 American Society for Microbiology. All Rights Reserved.



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