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Use of Yeast Populations Fractionated by Zonal Centrifugation to Study the Cell Cycle

Laboratory of Molecular Biology and Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706

ABSTRACT

Zonal centrifugation in a sucrose density gradient was used to separate yeast cells primarily by size and thus by age in the cell cycle. This approach provides an alternative to synchronous growth for examining the properties of cells at different stages in the cell cycle.

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• Yashphe, J, Halvorson, H. (1976). beta-D-galactosidase activity in single yeast cells during cell cycle of Saccharomyces lactis. Science 191: 1283-1284 [Abstract]