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J Bacteriol. 1971 December; 108(3): 1058-1064
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Detection of a Rapidly Metabolizing Portion of the Membrane Cardiolipin in Haemophilus parainfluenzae

Department of Biochemistry, University of Kentucky Medical Center, Lexington, Kentucky 40506

ABSTRACT

Heterogeneity in the metabolism of cardiolipin (CL) has been detected in Haemophilus parainfluenzae. Pulse-chase experiments showed that a portion of the total CL incorporated and then lost ³²P much more rapidly than the rest of the CL in the cells. The metabolism of each phosphate of the CL differed. The phosphate of the phosphatidyl glycerol (PG) portion of the CL had a more active metabolism than the phosphate of the phosphatidic acid portion of the molecule. Only a portion of the PG pool contributed to the formation of CL. Ethylenediaminetetraacetic acid inhibited the CL-specific phospholipase D in vitro and, when added to growing cells, resulted in more rapid PG metabolism, suggesting that CL hydrolysis contributed to the PG pool.