This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lambowitz, A. M. Articles by Slayman, C. W. Search for Related Content PubMed PubMed Citation Articles by Lambowitz, A. M. Articles by Slayman, C. W.

Cyanide-Resistant Respiration in Neurospora crassa

Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06510
Departments of Physiology and Microbiology, Yale University School of Medicine, New Haven, Connecticut 06510

ABSTRACT

Cell respiration in wild type and poky was studied as part of a long-term investigation of cyanide-resistant respiration in Neurospora. Respiration in wild type proceeds via a cytochrome chain which is similar to that of higher organisms; it is sensitive to antimycin A or cyanide. Poky, on the other hand, respires by means of two alternative oxidase systems. One of these is analogous to the wild-type cytochrome chain in that it can be inhibited by antimycin A or cyanide; this system accounts for as much as 15% of the respiration of poky f^– and 34% of the respiration of poky f⁺. The second oxidase system is unaffected by antimycin A or cyanide at concentrations which inhibit the cytochrome chain maximally. It can, however, be specifically inhibited by salicyl hydroxamic acid. The cyanide-resistant oxidase is not exclusive to poky, but is also present in small quantities in wild type grown under ordinary circumstances. These quantities may be greatly increased (as much as 20-fold) by growing wild type in the presence of antimycin A, cyanide, or chloramphenicol.

This article has been cited by other articles:

• Descheneau, A. T., Cleary, I. A., Nargang, F. E. (2005). Genetic Evidence for a Regulatory Pathway Controlling Alternative Oxidase Production in Neurospora crassa. Genetics 169: 123-135 [Abstract] [Full Text]  
• Funes, S., Nargang, F. E., Neupert, W., Herrmann, J. M. (2004). The Oxa2 Protein of Neurospora crassa Plays a Critical Role in the Biogenesis of Cytochrome Oxidase and Defines a Ubiquitous Subbranch of the Oxa1/YidC/Alb3 Protein Family. Mol. Biol. Cell 15: 1853-1861 [Abstract] [Full Text]  
• Borkovich, K. A., Alex, L. A., Yarden, O., Freitag, M., Turner, G. E., Read, N. D., Seiler, S., Bell-Pedersen, D., Paietta, J., Plesofsky, N., Plamann, M., Goodrich-Tanrikulu, M., Schulte, U., Mannhaupt, G., Nargang, F. E., Radford, A., Selitrennikoff, C., Galagan, J. E., Dunlap, J. C., Loros, J. J., Catcheside, D., Inoue, H., Aramayo, R., Polymenis, M., Selker, E. U., Sachs, M. S., Marzluf, G. A., Paulsen, I., Davis, R., Ebbole, D. J., Zelter, A., Kalkman, E. R., O'Rourke, R., Bowring, F., Yeadon, J., Ishii, C., Suzuki, K., Sakai, W., Pratt, R. (2004). Lessons from the Genome Sequence of Neurospora crassa: Tracing the Path from Genomic Blueprint to Multicellular Organism. Microbiol. Mol. Biol. Rev. 68: 1-108 [Abstract] [Full Text]  
• Medoff, G, Sacco, M, Maresca, B, Schlessinger, D, Painter, A, Kobayashi, G., Carratu, L (1986). Irreversible block of the mycelial-to-yeast phase transition of Histoplasma capsulatum. Science 231: 476-479 [Abstract]  
• Zelitch, I. (1975). Improving the Efficiency of Photosynthesis. Science 188: 626-633