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J Bacteriol. 1971 December; 108(3): 1135-1140
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Biosynthesis of 7,8-Diaminopelargonic Acid, a Biotin Intermediate, from 7-Keto-8-Aminopelargonic Acid and S-Adenosyl-L-Methionine¹

^a Department of Biochemistry, College of Physicians & Surgeons, Columbia University, New York, New York 10032

ABSTRACT

Resting cells of Escherichia coli strain D302(bioD302) can synthesize 7,8-diaminopelargonic acid from 7-keto-8-aminopelargonic acid. The product of this aminotransferase reaction has been identified by paper chromatography and electrophoresis. Glucose enhances the vitamer yield twofold. Of the 19 amino acids tested as amino donors, only methionine proved to be significantly stimulatory. In cell-free extracts, however, methionine was completely inactive unless both adenosine triphosphate (ATP) and Mg²⁺ were present. S-Adenosyl-L-methionine (SAM) was about 10 times more effective than methionine, ATP, and Mg²⁺. The optimal conditions for the reaction were determined, and substrate inhibition was found for 7-keto-8-aminopelargonic acid. It has been possible to eliminate certain impurities as amino donors in the commercial preparation of SAM and those that may arise in enzymatic reactions in which SAM is a substrate. The direct participation of SAM in the aminotransferase reaction seems a likely possibility.

² This work is taken from a dissertation to be submitted by G. L. Stoner in partial fulfillment of the requirements for the Ph.D. degree at Columbia University.

¹ Presented at the 62nd Annual Meeting of the American Society of Biological Chemists, San Francisco, Calif., 13 to 18 June 1971.

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