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Growth Characteristics of a Pseudomonad Which Utilizes Pyridoxine or Pyridoxamine as a Carbon Source

Department of Biochemistry, University of California, Berkeley, California 94720

ABSTRACT

Cultural characteristics of Pseudomonas MA-1, which has been extensively used as a source of enzymes that degrade vitamin B₆, confirm its classification as a pseudomonad. The organism requires biotin for growth; any one of several amino acids is also required for growth when pyridoxine (but not when glucose) serves as the energy source. Growth of Pseudomonas MA-1 is inhibited by one or more unidentified substances formed when pyridoxine is autoclaved with the medium. Modified cultural conditions are described which permit the large-scale growth of the organism with pyridoxine as carbon source.