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Mutants of Escherichia coli K-12 Blocked in the Final Reaction of Ubiquinone Biosynthesis: Characterization and Genetic Analysis

Department of Biochemistry, John Curtin School of Medical Research, The Institute of Advanced Studies, Australian National University, Canberra, A.C.T., Australia

ABSTRACT

The ubiquinone precursor 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1, 4-benzoquinone was isolated from two ubiquinone-deficient mutants of Escherichia coli and identified by nuclear magnetic resonance and mass spectrometry. The results of genetic analysis of the mutants indicate that each mutant carries a mutation in a gene designated ubiG which was located, by cotransduction with the nalA and glpT genes, at minute 42 on the E. coli chromosome.

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