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Nuclease Activities of Mycoplasma gallisepticum as a Function of Culture Age in Different Media¹

^a Biomolecular Hygiene Laboratories, Department of Animal Sciences, University of New Hampshire, Durham, New Hampshire 03824

ABSTRACT

Levels of deoxyribonuclease and ribonuclease activities in the supernatant (soluble plus ribosomes) fraction of Mycoplasma gallisepticum were assayed and found to be a function of strain, nutrient, and culture age. In yeast hydrolysate-enriched broth, maximal nuclease activities occurred during exponential growth.

² Present address: Department of Avian Diseases, New York State Veterinary College at Cornell University, Ithaca, N.Y. 14850.

¹ Part of a thesis submitted to the Graduate School of the University of New Hampshire in partial fulfillment of the requirements for the degree of Master of Science by the senior author. Published with the approval of the Director, New Hampshire Agricultural Experiment Station, as Scientific Contribution no. 542.