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J Bacteriol. 1972 January; 109(1): 81-88
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Studies on the Diversity of Inositol-Containing Yeast Phospholipids: Incorporation of 2-Deoxyglucose into Lipid

Department of Biochemistry, University of Kentucky, Lexington, Kentucky 40506

ABSTRACT

Seven inositol-containing phospholipids in Saccharomyces cerevisiae can be resolved by two-dimensional chromatography on silicic acid-impregnated paper. Four of these lipids are stable to mild alkaline methanolysis; one of these has already been characterized as a mannosyl di(inositolphosphoryl) ceramide. Addition of labeled 2-deoxy-D-glucose to a growing culture results in label appearing in five lipids, some of which are alkali-stable and have the same R_F values as the alkali-stable inositol-containing phospholipids. These lipids are labeled rapidly. The deoxyglucose is incorporated intact, probably in a glycosidic link, since the deoxyglucose is released by mild acid treatment.

¹ Present address: Department of Virology and Epidemiology, Texas Medical Center, Baylor College of Medicine, Houston, Texas 77025.

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